Standard M nCOV Real-Time PCR Detection Kit
STANDARD M nCoV Real-Time Detection kit is used for rapid identification and detection of novel coronavirus (2019-nCoV) nucleic acids in human nasopharyngeal swabs and throat swab samples. Coronavirus RNA was first transcribed into cDNA by reverse transcriptase, and then cDNA was used as a template for PCR amplification. The separation of the fluorophore and quencher makes the fluorescence signal detected by the instrument: FAM channel qualitative detection of the new coronavirus (COVID-19) ORF1ab (RdRp) gene, and JOE (VIC or HEX) channel qualitative detection of coronavirus) E gene, and CY5 channel detection internal reference.
Compatibility
- ̇LightCycler 480 (Roche)/li>
- ̇CFX96TM Dx System (Bio-Rad)
- ̇Applied Biosystems 7500 Real-Time PCR
Instrument System (Thermo Fisher Scientific)
Applications
- ̇Nasopharyngeal swab
- ̇Oropharyngeal swab
- ̇Sputum
Key Components
| Components | Quantity | Test Dosage in each reaction | |
|---|---|---|---|
| 1 | 2019-nCoV Reaction Solution | 750μl / vial x 2 | 14μl |
| 2 | RTase Mix | 630μl / vial x 1 | 6μl |
| 3 | 2019-nCoV Positive control | 600μl / vial x 1 | – |
| 4 | Negative control | 600μl / vial x 1 | – |
| 5 | Internal control A | 525μl / vial x 1 | 5μl (Add with specimen) 0.5μl (Amplification directly) |
| 6 | ROX | 55μl / vial x 1 | 0.5μl (for Applied Biosystems 7500) |
Ordering Information
| Cat No. | Product | Size |
|---|---|---|
| 11NCO10 | STANDARDM nCoV Real-Time Detection kit | 96T |
Benefits
| Fast & Easy | High Sensitivity & Specificity |
|---|---|
| ̇One tube reaction for identification and detection of 2019-nCoV ̇One-step Real-Time RT-PCR ̇Provide all reagents required for PCR |
̇Designed according to “WHO interim guidance for laboratory testing for 2019 novel coronavirus (2019-nCoV) in humans” ̇nCoV primers/probes ORF1ab (RdRp) gene, E gene ̇Provide Internal controls |